Product Info Summary
SKU: | MP00241 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody
SKU/Catalog Number
MP00241
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody catalog # MP00241. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00241)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbH41
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Histone H2A.X (S139)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
MP00241 is reactive to H2AX in Human, Mouse, Rat
Applications
MP00241 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
15 kDa
Calculated molecular weight
15.145kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HistoneH2A.X antigen affinity purified monoclonal antibody (Catalog # MP00241) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HistoneH2A.X at approximately 15 kDa. The expected band size for HistoneH2A.X is at 15 kDa.
Click image to see more details
Figure 2. IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241).
HistoneH2A.X was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241).
HistoneH2A.X was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241).
HistoneH2A.X was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241).
HistoneH2A.X was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis of HeLa cells treated with H2O2, using Phospho-Histone H2A.X (S139) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For H2AX (Source: Uniprot.org, NCBI)
Gene Name
H2AX
Full Name
Histone H2AX
Weight
15.145kDa
Superfamily
histone H2A family
Alternative Names
H2A histone family, member X; H2A.X; H2a/x; H2AX histone; H2AXhistone H2A.x; YH2AX H2AX H2A.X, H2A/X, H2AFX H2A.X variant histone histone H2AX|H2A histone family member X|H2AX histone|histone H2A.x
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H2AX, check out the H2AX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H2AX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody (MP00241)
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